Core Laboratory

16. PAI

The PAI assay is based on two stages:

In Stage One, a fixed amount of tPA is added to the samples and allowed to react with the PAI present in the plasma sample. The tPA must be in excess of the PAI. Then the sample is acidified in order to destroy alpha-2-antiplasmin, which will otherwise interfere with the following tPA assay.

In Stage Two, the residual tPA activity is measured. The PAI content is then identified as the difference between the amount of tPA activity added and the amount of residual tPA activity found. One chain tPA is used to make the assay specific for PAI-1 activity.

Reference:

  1. Ranby, M; Norman, B, Wallen P. A sensitive assay for tissue plasminogen activator. Thromb Res 27:743-749, 1982

 

For more information, please contact the Core Laboratory manager or director.