The procedure is a sequential radioimmunoassay. After preincubation of the patient sample with anti-glucagon antibody, 125I-labeled glucagon competes with glucagon in the sample for antibody sites. After incubation for a fixed time, separation of bound from free is achieved by the PEG-accelerated double-antibody method, followed by centrifugation. The precipitate containing the antibody-bound fraction is then counted, and patient concentrations are read from a calibration curve.
- Nishino, T et al: Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment. Clinical Chemistry 27: 1690-97, 1981.
- Unger, RH; Orci, L: Glucagon secretion and metabolism in man. Endocrinology, L.J. DeGroot ed, Volume Two, 1318-32, (Philadelphia: W.B. Saunders, 1989).
Interassay Coefficient of Variation (CV):
Sample 1 = 15.7% (n=20, mean=37, sd=5.8)
Sample 2 = 6.5% (n=20, mean=159, sd=10.4)
Sample 3 = 5.7% (n=20, mean=534, sd=30.6)
For more information, please contact the Core Laboratory manager or director.