Core Laboratory
6. Growth Hormone (Human)
The assay is a two-site immunoradiometric assay using two different antibodies in excess. During incubation, human growth hormone in the sample reacts with 125I-anti-human growth hormone antibodies and unlabelled anti-human growth hormone antibodies directed against a separate epitope. Bound and free 125I-anti-human growth hormone are separated by centrifugation and decanting. The radioactivity is directly proportional to the concentration of human growth hormone in the sample.
References:
- Livesey, JH, Hodgkinson SC, Roud HR and Donald RA: Clin Biochem 13: 151-155, 1980.
- Friedman RB. Effects of Disease on Clinical Laboratory Test. Second ed. AACC Press : chapter 3, 115-116, 1989.
- Tryding N et al. Drug Effects in Clinical Chemistry. Stockholm: Apoteksbolaget :367, 1992.
Interassay Coefficient of Variation (CV):
Sample 1 = 5.2% (n=20, mean=91.05, sd=4.69)
Sample 2 = 4.0% (n=20, mean=579.39, sd=22.90)
Sample 3 = 6.4% (n=20, mean=993.24, sd=63.46)
Detection Limit:
28 nmol/L
For more information, please contact the Core Laboratory manager or director.