Core Laboratory
8. Tumor Necrosis Factor-a (TNF-a) Human
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-a has been pre-coated onto a microtiter plate. Standards and samples are pipetted into the wells and any TNF-a present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for TNF-a is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. After an incubation period, an amplifier solution is added to the wells and color develops in proportion to the amount of TNF-a bound in the initial step. The color development is stopped and the intensity of the color is measured.
References:
- Tumor Necrosis Factor: Structure, Function and Mechanism of Action. Aggarwal, B.B. and J. Vilcek, eds. Marcek Dekker, Inc. (1991).
- Vilcek, J. and T.H. Lee J. Biol. Chem. 266:7313-7316. (1991).
Interassay Coefficient of Variation (CV):
Sample 1 = 10.4% (n=20, mean=2.4, sd=0.25)
Sample 2 = 7.5% (n=20, mean=15.41, sd=1.16)
Sample 3 = 7.8% (n=20, mean=32.34, sd=2.53)
Detection Limit:
0.5 pg/mL
For more information, please contact the Core Laboratory manager or director.