Core Laboratory
9. Glucose
Procedure:
Glucose HK Liquid(GLUCL)
Principle:
Enzymatic reference method with hexokinase. Glucose is oxidized by glucose oxidize in the presence of oxygen and water to produce laconic acid and hydrogen peroxide. The rate of uptake of oxygen from its solution in the reagent is determined by a polar graphic electrode at its maximum value and compared with the rate for a standard stored as a calibration factor.
Specimen:
- Serum or heparinized plasma in serum or plasma separator tubes or plasma in grey top tube (fluoride/oxalate). Acceptable anticoagulants are heparin, EDTA and fluoride. Red & green top microtainers are also acceptable.
- Separate serum immediately serum from clot as average decrease is approx. 7% in 1 hour (0.28 - 0.56 mmol/L).
- Stability: Unhemolysed serum 8 hrs at room temperature 25 degrees Celsius, or 3 days at 4 degrees Celsius.
Instrument:
Cobas Integra
Calibration:
Units: mmol/L. Two point calibration in duplicate.
Data Analysis:
Linear regression.
QC:
2 levels
Interference:
Lipemia - no significant interference
Hemolysis - no significant interference
Icterus - no significant interference
Reference Range:
Fasting Glucose: 4.0 - 6.0 mmol/L
Post-prandial Glucose: 8.0 mmol/L
Random Glucose: 4.0 - 8.0 mmol/L
For more information, please contact the Core Laboratory manager or director.